The Weeden House Museum is a historic house museum located in Huntsville, Alabama. It is best noted for its connection with Maria Howard Weeden, a female poet and artist who became known during the late 19th century for her watercolor portraits of her former slaves.

The Weeden House was built in 1819, the same year that Alabama was made into a state, by Henry C. Bradford. It was made in the Federal style and was purchased in 1845 by Dr. William Weeden. The house stayed in the family until 1950, when it was sold out of the family.

The Weeden House Museum is now a part of the Twickenham Historic District. It features many 19th century pieces and items from the Weeden family.