The Chassahowitzka National Wildlife Refuge is a nature preserve area in Crystal River, Florida. It is best known for being the wintering site of the eastern population of the Whooping Cranes.

The Chassahowitzka National Wildlife Refuge was established in the early 1940s to serve as a waterfowl sanctuary. Its more than 31,000 acres of area with saltwater bays, brackish marshes and estuaries have always been inviting to migrating wildlife, so from being a waterfowl habitat, it is now also home to the endangered West Indian manatees, some alligators, river otter, green turtles, and gopher tortoises. There is also tons of other wildlife in the area like cormorants, herons, raccoons, deer, black bear, bobcats, ospreys, pelicans, ducks, and songbirds just to name a few.