The Crowley Museum & Nature Center is an educational center in Sarasota, Florida. It is a mix of local history and nature, presenting both the excellent features of the Southwest Florida history and scenery.

The Crowley Museum and Nature Center was established in 1974 as the Nature Center and Pioneer Museum by William Jasper Crowley. It was later renamed after its founder, who has helped in preserving the history and environment of the rural region.

The Crowley Museum & Nature Center presents 190 acres of nature and history. It has three miles of nature trails, boardwalks, and a number of historic structures including an 1892 Cracker House, cabin, and blacksmith shop. The site is open year round in Thursdays through Sundays from 10 am to 4 pm.