The International Trolley & Train Museum is a railroading history museum in Orlando, Florida. It features displays that tell of the importance of the said industry in the history and development of the United States.

The International Trolley & Train Museum features 14 model railroad trains that are housed in an indoor garden. The site takes visitors around in a mix of train models and a replicated nature walk with waterfalls, mountains, tunnels and trestles. The trains are mostly from the 1920s to the present. Their main highlights are the California Victorian style half open, half closed trolley and the 5/8 scale replica of an 1880 Mason Bogey locomotive with passenger cars.

The International Trolley & Train Museum is ranked 59 out of 264 things to do in Orlando, as according to Lonely Planet.