The Knott House Museum is a historic house museum in Tallahassee, Florida. It is best noted as one of the most historic structures in the city.

Located in the noted Park Avenue Historic District, the Knott House Museum features the 1843 William V. Knott House. It was built for attorney Thomas Hagner and wife Catherine Gamble, but the house was best known for being the residence of the Knott family, who were noted for their influence at most. The father, William Knott, was a politician (he was the first state financial agent), while his wife, Luella, was an artist.

Aside from the Knotts, the Knott House also served as the home of Dr. George Betton (who trained the first African American physician in the state), as well as three other Florida Supreme Court judges.