The Margaret Kampsen Historic Dundee Depot Museum is a local history museum in Dundee, Florida. It tells of the general history of the area, which is about everything that has helped shape the town that is Dundee today.

Opened in 1998, the Margaret Kampsen Historic Dundee Depot Museum is housed in the historic Atlantic Coast Line Railroad depot. It was built in 1912 and helped bring in settlers to the area and has been recently listed in the National Register of Historic Places. It features several displays that tell the humble beginnings and development of the locale.

Originally known as the Dundee Depot Museum, the Margaret Kampsen Historic Dundee Depot was renamed in 2009 after Margaret Kampsen. The site is open on Thursdays and Saturdays from 10 am to 3 pm.