The Naples Depot Museum is a railroading history museum in Naples, Florida. It tells of the colorful railroading past of the city of Naples and how the industry has helped in the development and growth of the area.

Naples has been one of the overlooked areas up until the 1880s, when two rival railroading companies entered the area. From being a remote settlement with only 300 residents, the community quickly boomed soon enough, leading to the Naples that people know today.

In celebration of this part of the city’s history, the Naples Depot Museum was established. It is housed in the historic 1928 Seaboard Air Line depot and features various displays related to the early transportation means of the locals as well as railroading.