The Polk County Historical Museum is a local history museum in Bartow, Florida. It houses displays that tell of the local and regional history.

The Polk County Historical Museum opened in 1998. It is housed in the historic 1909 Old Polk County Courthouse (which is also known as the Imperial Polk County Courthouse). Through its exhibits about the various areas and facets of the life in Polk County and Central Florida, it manages to tell of the story of the region, with special focuses on pioneers, military, community, environment, and overall industry of the locale.

Aside from the displays, the Polk County Historical Museum also offers facility rentals for various events and gatherings. Two restored courtrooms are available for rent.