The Tallahassee Museum is regional museum located in Tallahassee, Florida. This museum is dedicated to the natural and cultural history of Tallahassee and the surrounding area, showcasing historical artifacts from the 19th century to recent times.

Founded in 1957 as the Tallahassee Junior Museum, the Tallahassee museum is unique among other regional museums as it is located in a vast fifty-two acre property which is used to features interesting exhibits in their natural locales and environments.

A large portion of the site is dedicated to a recreation of Florida rural lifestyle and agricultural practices. This includes a recreation of period farmhouses from the 19th century, grist mills and even a turpentine commissary. Another major area include wildlife exhibits that feature local animals in their natural habitat.