The West Florida Railroad Museum is a railroading history museum in Milton, Florida. It is dedicated to preserving, chronicling, and showcasing the railroading heritage of Northwest Florida.

The West Florida Railroad Museum tells of the great growth that the railways help achieve in the area. The site most primarily tells of the story of the establishment of the Pensacola and Atlantic Railroad, which was built in 1882 to connect Pensacola with the other parts of the state. Prior to the 1880s, the beach haven Pensacola was only reachable through water travel or by trekking the Florida wilderness. The railway provided an easy access to the said tourist destination, and also ushered in tons of opportunities to the areas that are on the way to it.