The Zephyrhills Depot Museum is a railroading history museum in Zephyrhills, Florida. It features the city of Zephyrhills? old depot and tells of its impact in the local history and economy.

Housed in a 1927 Atlantic Coast Line Railroad Depot, the Zephyrhills Depot Museum tells of the history of trains and the city of Zephyrhills. It features displays like artifacts, memorabilia, and photographs that tell of the city?s railroading history. Opened in 1998, it has four exhibit wings focused on various themes and topics. The Abbott Station Room tells of the city?s colorful past, the Zephyrhills Alumni Room is for school memorabilia, the Community Room is all about community heritage, and the Depot Room features artifacts and historic items from the original depot.