The Ste. Anne de Detroit Church is the second oldest operational Roman Catholic church in the United States. This red church is located at 1000 Ste. Anne Street in Detroit, Michigan. This church was the first building constructed in the historic French settlement of Fort Pontchartrain du Detroit, the predecessor of the present city. Cadillac and French colonist arrived via the Detroit River on July 24, 1701. The church and parish was founded and named in honor of Saint Anne, patron saint of France.

The church is notable for its architecture. Leon Conquard and Alert E. French designed the church using Classic and Gothic Revival styles, employing a distinctively French feature, the flying buttresses. The church possesses the oldest stained glass artwork in the city. The church stands in front of a landscaped brick and tree-lined plaza. The church was added to the National Register of Historic Places in 1976.