The No Man?s Land Museum houses a number of permanent exhibits detailing the history of No Man?s Land. The Museum also contains 4 rooms that are dedicated to items that are used in the daily lives of the early ranchers and homesteaders, those who witnessed the emergence of modern Panhandle, and those who surpassed the Dust bowl and the Depression. Items in these rooms include antique quilts, horse drawn hearse and freight wagons, and a large barbed wire collection.

There is also a 5th room that showcases chipped stone tools, pottery, grinding stones, and other items that are used in more ancient lifeways of Native Americans in the region. It also houses an extensive collection of Plains, arrowheads, and fine examples of beadwork and porcupine quillwork.