The Middleburg Plantation is a historic site in Berkeley County, South Carolina, just outside of the city of Charleston. It was established around the late 1690s by a French Huguenot planter named Benjamin Simmons. The site served as a rice plantation for a very long time.

Initially composed of just 100 acres, the Middleburg Plantation grew to 3000 acres at its peak in 1772. It is also known to be the home of the oldest surviving wooden plantation house in the state of South Carolina. The dwelling is a six bedroom house and is believed to be virtually unchanged since the 1800s. The Middleburg Plantation garden is also known to be the oldest original garden in the country, as it has been laid out around the early 1800s.