The Karl C. Harrison Museum of George Washington is a museum in Columbiana, Alabama focused on the colonial period of the United States. It is also noted for having the larger privately owned collections of artifacts related to George and Martha Washington.

Established in 1982, the Karl C. Harrison Museum of George Washington is named after its founder, Karl C. Harrison, a Columbiana banker and philanthropist. He started the museum with the help and encouragement of descendants of Martha Washington, Eliza Parke Curtis and Charlotte Smith-Weaver. The ladies donated heirlooms and other artifacts, which served as the initial collection of the museum.

The Karl C. Harrison Museum of George Washington features a number of important items like letters, furniture, paintings, glassware, and jewelry from the colonial period until 1865. Other valuable items are also found on the site.